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M94A2735.TXT
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1994-10-25
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Document 2735
DOCN M94A2735
TI Cell-associated and extracellular HIV-1 antigens: variations in
serologic enzyme-immuno assays (EIAs).
DT 9412
AU Troshev O; Argirova R; Central AIDS Lab., Sofia, Bulgaria.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):237 (abstract no. PB0377). Unique
Identifier : AIDSLINE ICA10/94369840
AB OBJECTIVES: To investigate the influence of HIV-1 antigen source and
preparation upon signal-to-background ratio in three different EIAs; and
determine how Western blot banding pattern variations reflect virus
glycoprotein processing pathways. METHODS: Both group of antigens for
the EIAs (ELISA, Dot-ELISA and confirmatory Western blot) were prepared
from HIV-1 producing cell line HUT/ARV4, using various approaches and
biochemical procedures. RESULTS: We have achieved the optimal
signal-to-background ratio in these EIAs as follows: 1) ELISA with
extracellular HIV-1 antigen purified in a sucrose density gradient; 2)
Dot-ELISA with extracellular antigen precipitated with PEG-6000; 3)
Western blot (WB) with cell-associated HIV-1 lysate-homogenate. In last
case the envelope precursor polyprotein gp160 has regularly appeared
along with its cleavage products (gp120 and gp41), whereas a WB with
extracellular virus has always reflected gp120 and gp41 only.
CONCLUSIONS: The extracellular HIV antigen is more appropriate in a
screening serology (ELISA or Dot-ELISA) because of significantly reduced
non-specific background. The cell-associated HIV-1 antigen in Western
blot enables to detect precisely all the three envelope-specific
antibodies, directed to the precursor, external and transmembrane
glycoproteins of HIV-1.
DE Blotting, Western Comparative Study Enzyme-Linked Immunosorbent
Assay/METHODS Gene Products, env/BLOOD Human HIV Antigens/*BLOOD HIV
Envelope Protein gp120/BLOOD HIV Envelope Protein gp41/BLOOD HIV
Seropositivity/*DIAGNOSIS/IMMUNOLOGY HIV-1/*IMMUNOLOGY *Immunoenzyme
Techniques Predictive Value of Tests Protein Precursors/BLOOD MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).